The effects of micronutrient-enriched media on the efficacy of Bacillus subtilis as biological control agent against Meloidogyne incognita

The root-knot nematodes Meloidogyne incognita poses a serious threat to horticultural food and plantation crops. One of the effective efficient measures overcome this nematode is using bacteria as biological control agent. This study aimed test effectiveness Bacillus subtilis grown on media with additional micronutrients agent for M. incognita. was carried out by growing in 100 mL nutrient broth (NB) added FeCl2, MnCl2, CuCl2. Each type micronutrient administered at concentrations 35 ppm, 40 45 50 55 ppm. As control, NB used without addition micronutrients. results showed that best performances bacterial growth were ppm CuCl2, MnCl2. These six treatments then an antagonism test. A total 150 J2 4 suspension put into petri dish diameter 5 cm. After that, 1 each following treatment. mortality rate treatment 77.5% 74.5% 77.25% 73.25% 78.75% 79% 53.25% control. same also measure chemotaxis index tomato roots. Tomato seeds Tantyna variety soaked 60 minutes micronutrient-enriched media. seven days, seed roots removed placed 20% Pluronic F-127 Gel which had been infested 6 hours, number approaching observed. ranged from 16% 24%, while 33%. corroborates MnCl2 CuCl2 medium B. can increase antagonistic activity against suppress its response suspension.